GRP78/BIP Monoklonaler Antikörper

GRP78/BIP Monoklonal Antikörper für FC (Intra), WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen

1

CloneNo.

1D6F7

Kat-Nr. : CL594-66574

Synonyme

BIP, FLJ26106, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSPA5, MIF2

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Galerie der Validierungsdaten

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  WB analysis using CL594-66574

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-66574 (GRP78/BIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using CL594-66574

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GRP78/BIP (CL594-66574, Clone:1D6F7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66574 bindet in WB, FC (Intra) GRP78/BIP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GRP78/BIP fusion protein Ag2188
• Vollständiger Name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC020235
• Gene symbol: HSPA5
• Gene ID (NCBI): 3309
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.

Publikationen

Species	Application	Title
human		Exp Cell Res; BFGF attenuates aortic valvular interstitial cell calcification by inhibiting endoplasmic reticulum stress-mediated apoptosis; Authors - Gao Yuan

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C