NMDAR2B/GRIN2B Monoklonaler Antikörper

NMDAR2B/GRIN2B Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C5E12

Rezensionen (1)

Kat-Nr. : 66565-1-Ig

Synonyme

GluN2B, GRIN2B, hNR3, NMDAR2B, NMDAR2B/GRIN2B, NR2B, NR3

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 66565-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66565-1-Ig (NMDAR2B/GRIN2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66565-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66565-1-Ig (NMDAR2B/GRIN2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66565-1-Ig bindet in WB, ELISA NMDAR2B/GRIN2B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NMDAR2B/GRIN2B fusion protein Ag16718
• Vollständiger Name: glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• Berechnetes Molekulargewicht: 1484 aa, 166 kDa
• Beobachtetes Molekulargewicht: 166 kDa
• GenBank-Zugangsnummer: BC113620
• Gene symbol: GRIN2B
• Gene ID (NCBI): 2904
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRIN2B (also known as GluN2B or NMDAR2B) is a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. NMDA receptors are widely expressed in the central nervous system and play a major role in excitatory synaptic transmission and plasticity (PMID: 23223336). NMDA receptors large multi-subunit complexes arranged into heteromeric assemblies composed of four homologous subunits within a repertoire of over 10 different subunits: eight GluN1 isoforms, four GluN2 subunits (A-D) and two GluN3 subunits (A and B) (PMID: 21395862). Naturally occurring mutations within GRIN2B gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lisa (Verified Customer) (10-04-2021)

The antibody signal is not very strong and specific on hiPSCs derived neurons: it is detectable with an exposure of about 90 seconds, but sometimes gives aspecific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSCs-derived neurons