NMDAR1 Polyklonaler Antikörper

NMDAR1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 27676-1-AP

Synonyme

GluN1, GRIN1, NMDAR1, NR1

---

Galerie der Validierungsdaten

• 

  WB analysis using 27676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27676-1-AP (NMDAR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 27676-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27676-1-AP (NMDAR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27676-1-AP (NMDAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27676-1-AP (NMDAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27676-1-AP (NMDAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27676-1-AP (NMDAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Produktinformation

27676-1-AP bindet in WB, IP, IHC, ELISA NMDAR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NMDAR1 fusion protein Ag26364
• Vollständiger Name: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: NM_000832
• Gene symbol: GRIN1
• Gene ID (NCBI): 2902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission; Authors - Pengcheng Ma
rat	WB	Aging (Albany NY); EGB761 ameliorates chronic cerebral hypoperfusion-induced cognitive dysfunction and synaptic plasticity impairment.; Authors - Zhao-Hui Yao
rat	WB,IP	Heliyon; Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1; Authors - Chuan Jiang
human	WB,IHC	Life Sci; Identification of a robust scoring system based on metabolic genes followed by in-depth validation of ATP1A3 in glioma; Authors - Qiankun Ji
mouse	IHC	Eur J Pharmacol; Glutamate ionotropic receptor NMDA type subunit 1: A novel potential protein target of dapagliflozin against renal interstitial fibrosis; Authors - Yuyuan Liu