GRIA4 Polyklonaler Antikörper

GRIA4 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 23350-1-AP

Synonyme

GluA4, GluR 4, GluR D, GLUR4, GLUR4C, GLURD, Glutamate receptor 4, GRIA4

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Galerie der Validierungsdaten

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  WB analysis of rat brain using 23350-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23350-1-AP (GRIA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 23350-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23350-1-AP (GRIA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 23350-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 23350-1-AP (GRIA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 23350-1-AP

  IP Result of anti-GRIA4 (IP:23350-1-AP, 4ug; Detection:23350-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 23350-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23350-1-AP (GRIA4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 23350-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23350-1-AP (GRIA4 Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of SH-SY5Y using 23350-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug GRIA4 antibody (23350-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Maus-Cerebellum-Gewebe, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

23350-1-AP bindet in WB, IP, IHC, FC, ELISA GRIA4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRIA4 fusion protein Ag19949
• Vollständiger Name: glutamate receptor, ionotrophic, AMPA 4
• Berechnetes Molekulargewicht: 902 aa, 101 kDa
• Beobachtetes Molekulargewicht: 101 kDa
• GenBank-Zugangsnummer: BC167786
• Gene symbol: GRIA4
• Gene ID (NCBI): 2893
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin
human	WB	J Clin Invest; TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.; Authors - Haiqing Zhang
mouse	IP	Cell Death Dis; ZDHHC8 critically regulates seizure susceptibility in epilepsy.; Authors - Qin Yang
human	WB	Front Cell Dev Biol; Olfactomedin-3 Enhances Seizure Activity by Interacting With AMPA Receptors in Epilepsy Models.; Authors - Shirong Tang
mouse	WB	Mol Brain; Assessments of prolonged effects of desflurane and sevoflurane on motor learning deficits in aged AppNL-G-F/NL-G-F mice.; Authors - Ryo Niikura
rat	WB	J Psychiatr Res; Hippocampal AMPA receptors mediate the impairment of spatial learning and memory in prenatally stressed offspring rats.; Authors - Dan Yao

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