GRIA1 Monoklonaler Antikörper

GRIA1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1F4A1

Rezensionen (2)

Kat-Nr. : 67642-1-Ig

Synonyme

GluA1, GLUH1, GluR 1, GluR A, GluR K1, GLUR1, GLURA, Glutamate receptor 1, GRIA1, HBGR1

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Galerie der Validierungsdaten

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  WB analysis using 67642-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spinal cord using 67642-1-Ig

  pig spinal cord tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig cerebellum using 67642-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67642-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67642-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67642-1-Ig (GRIA1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67642-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67642-1-Ig (GRIA1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 67642-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GRIA1 antibody (67642-1-Ig, Clone: 1F4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 67642-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GRIA1 antibody (67642-1-Ig, Clone: 1F4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Hausschwein-Cerebellum-Gewebe, Hausschwein-Hirngewebe, Hausschwein-Rückenmarkgewebe, Kaninchenhirngewebe, Maus-Cerebellum-Gewebe, Maushirngewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:5000-1:20000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

67642-1-Ig bindet in WB, IP, IHC, IF, ELISA GRIA1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GRIA1 fusion protein Ag28932
• Vollständiger Name: glutamate receptor, ionotropic, AMPA 1
• Berechnetes Molekulargewicht: 906 aa, 102 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC111734
• Gene symbol: GRIA1
• Gene ID (NCBI): 2890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	CNS Neurosci Ther; Integrin α3 is required for high-frequency repetitive transcranial magnetic stimulation-induced glutamatergic synaptic transmission in mice with ischemia; Authors - Li Liu
rat	WB	Exp Neurol; Long-term variable photoperiod exposure impairs the mPFC and induces anxiety and depression-like behavior in male wistar rats; Authors - YuanYuan Hou
mouse	WB	Phytother Res; Rapid antidepressant-like effect of Fructus Aurantii depends on cAMP-response element binding protein/Brain-derived neurotrophic facto by mediating synaptic transmission.; Authors - Lei Wu
rat	WB	J Psychiatr Res; Hippocampal AMPA receptors mediate the impairment of spatial learning and memory in prenatally stressed offspring rats.; Authors - Dan Yao
mouse	WB	Cell Biosci; Amelioration of olfactory dysfunction in a mouse model of Parkinson's disease via enhancing GABAergic signaling; Authors - Xing-Yang Liu
mouse	WB,IF,IP	CNS Neurosci Ther; Inhibition of ANXA2 activity attenuates epileptic susceptibility and GluA1 phosphorylation; Authors - Limin Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Pegah (Verified Customer) (02-03-2023)

I used a concentration of 1:500 of GRIA1 Monoclonal antibody (67642-1-Ig) for paraformaldehyde fixed mouse brain tissue with 40um diameter.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

GRIA1 Antibody Immunofluorescence validation (1:500 dilution) in Mouse brain (Cat no:67642-1-Ig)

FH

Lisa (Verified Customer) (10-04-2021)

The antibody signal is very strong and clean. Highly recommended.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSCs-derived neurons