GRHPR Polyklonaler Antikörper

GRHPR Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 51013-2-AP

Synonyme

GLXR, GLYD, GRHPR, PH2

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 51013-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 51013-2-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 51013-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 51013-2-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 51013-2-AP (GRHPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 51013-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51013-2-AP (GRHPR Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 51013-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51013-2-AP (GRHPR Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary tumor using 51013-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51013-2-AP (GRHPR antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, humanes Lebergewebe, L02-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

51013-2-AP bindet in WB, IHC,ELISA GRHPR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRHPR fusion protein Ag0484
• Vollständiger Name: glyoxylate reductase/hydroxypyruvate reductase
• Berechnetes Molekulargewicht: 328 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC000605
• Gene symbol: GRHPR
• Gene ID (NCBI): 9380
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRHPR(Glyoxylate reductase/hydroxypyruvate reductase) is also named as GLXR and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The GRHPR gene encodes a predicted 328-amino acid protein with a calculated molecular mass of 35.5 kD. It has glyoxylate reductase (GR), hydroxypyruvate reductase (HPR) and D-glycerate dehydrogenase (DGDH) activities(PMID:16597637). Defects in GRHPR are the cause of hyperoxaluria primary type 2 (HP2)(PMID: 10484776). It can exsit as a homodimer(PMID:16756993).