GPX8 Polyklonaler Antikörper

GPX8 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 16846-1-AP

Synonyme

GPx 8, GPX8, GSHPx 8, UNQ847

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Galerie der Validierungsdaten

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  WB analysis of U-251 using 16846-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 16846-1-AP (GPX8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16846-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16846-1-AP (GPX8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16846-1-AP

  IP Result of anti-GPX8 (IP:16846-1-AP, 4ug; Detection:16846-1-AP 1:300) with HEK-293 cells lysate 3000ug.

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  IHC staining of human stomach cancer using 16846-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16846-1-AP (GPX8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 16846-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16846-1-AP (GPX8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-251-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

16846-1-AP bindet in WB, IP, IHC, IF, ELISA GPX8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPX8 fusion protein Ag9930
• Vollständiger Name: glutathione peroxidase 8 (putative)
• Berechnetes Molekulargewicht: 209 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC029424
• Gene symbol: GPX8
• Gene ID (NCBI): 493869
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPX8(Probable glutathione peroxidase 8) is also named as GSHPx-8 and belongs to the glutathione peroxidase family. Glutathione peroxidase (GPx) reduces hydroperoxides, including hydrogen peroxides, in the presence of reduced glutathione as a means of protecting organisms from oxidative damage. Several GPx isozymes have been identified in animal cells and these have been classified into different groups according to their cellular location and substrate specificity. In humans, eight types of GPx have been identified from GPx1 to GPx8. The full length GPX8 has 209 amino acids and the molecular weight is 24 kDa.

Publikationen

Species	Application	Title
human	WB	J Med Virol; SARS-CoV-2 nucleocapsid protein enhances the level of mitochondrial reactive oxygen species; Authors - Haiyun Yu
rat	WB,IF	Free Radic Biol Med; ER-resident antioxidative GPx7 and GPx8 enzyme isoforms protect insulin-secreting INS-1E β-cells against lipotoxicity by improving the ER antioxidative capacity.; Authors - Ilir Mehmeti
human	IHC	Medicina (Kaunas); Chosen Antioxidant Enzymes GPx4 and GPx8 in Human Colorectal Carcinoma: Study of the Slovak Population.; Authors - Andriana Pavliuk-Karachevtseva
human	WB,IHC	MedComm (2020); Glutathione peroxidase 8 expression on cancer cells and cancer-associated fibroblasts facilitates lung cancer metastasis; Authors - Yu‐Lian Xu ; KD Validated
rat	WB	FASEB J; Luminal H(2) O(2) promotes ER Ca(2+) dysregulation and toxicity of palmitate in insulin-secreting INS-1E cells; Authors - Sarah Sharifi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Andrea (Verified Customer) (01-23-2023)

Sharp and strong signal at 24 kDa with clear background.

• Applications: Western Blot