GPX5 Polyklonaler Antikörper

GPX5 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 18731-1-AP

Synonyme

EGLP, Glutathione peroxidase 5, GPx 5, GPX5, GSHPx 5

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 18731-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18731-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 18731-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18731-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 18731-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18731-1-AP (GPX5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 18731-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18731-1-AP (GPX5 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, humanes Hodengewebe, Mauslebergewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 1 publications below

Produktinformation

18731-1-AP bindet in WB, IHC, IF, ELISA GPX5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: glutathione peroxidase 5 (epididymal androgen-related protein)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 22-28 kDa
• GenBank-Zugangsnummer: NM_001509
• Gene symbol: GPX5
• Gene ID (NCBI): 2880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPX5 belongs to the glutathione peroxidase family. GPX5 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Catalytic the reaction: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. GPX5 constitute a glutathionine peroxidase-like protective system against peroxide damage in sperm membrane lipids. GPX5 has a calculated molecular mass of 25 kDa and apparent molecular mass of 22-28 kDa due to the cleavage of signal peptide and glycosylation.

Publikationen

Species	Application	Title
goat	WB	Oxid Med Cell Longev; Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway.; Authors - Tian-Yu Feng
mouse	IF	Sci Rep; Proteasome activators, PA28γ and PA200, play indispensable roles in male fertility.; Authors - Lin Huang
mouse	WB	Mol Biol Rep; Autophagy-related 7 proteindependent autophagy mediates resveratrol-caused upregulation of mitochondrial biogenesis and steroidogenesis in aged Leydig cell; Authors - Fanhong Lin
pig	WB	Animals (Basel); Effect of Boar Sperm Proteins and Quality Changes on Field Fertility.; Authors - Ilias Michos
pig	WB	Theriogenology; Effects of kojic acid on boar sperm quality and anti-bacterial activity during liquid preservation at 17 C.; Authors - Weike Shaoyong
human	WB	Anim Reprod Sci; Triosephosphate isomerase (TPI) and epididymal secretory glutathione peroxidase (GPX5) are markers for boar sperm quality.; Authors - Ingrid Vilagran