GPX4 Polyklonaler Antikörper

GPX4 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

121

Kat-Nr. : 14432-1-AP

Synonyme

GPX4

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Galerie der Validierungsdaten

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  WB analysis using 14432-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14432-1-AP (GPX4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 14432-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPX4 antibody (14432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 110 publications below
• IHC: See 23 publications below
• IF: See 17 publications below
• CoIP: See 2 publications below

Produktinformation

14432-1-AP bindet in WB, IHC, IF, CoIP, ELISA GPX4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPX4 fusion protein Ag5809
• Vollständiger Name: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 20-23 kDa
• GenBank-Zugangsnummer: BC022071
• Gene symbol: GPX4
• Gene ID (NCBI): 2879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

Publikationen

Species	Application	Title
mouse	WB	Biomaterials; Tumor microenvironment-initiated lipid redox cycling for efficient triple-negative breast cancer therapy; Authors - Tian-Jiao Zhou
chicken	WB	J Hazard Mater; Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers; Authors - Gaolong Zhong
mouse	IHC	Drug Des Devel Ther; Vancomycin Induced Ferroptosis in Renal Injury Through the Inactivation of Recombinant Glutathione Peroxidase 4 and the Accumulation of Peroxides; Authors - Xuedong Yin
mouse	WB	Drug Des Devel Ther; Ketamine Attenuates Airway Inflammation via Inducing Inflammatory Cells Apoptosis and Activating Nrf2 Pathway in a Mixed-Granulocytic Murine Asthma Model; Authors - Shilin Xiao
mouse	WB	Mater Today Bio; In situ injectable hydrogel-loaded drugs induce anti-tumor immune responses in melanoma immunochemotherapy.; Authors - Jiehan Li
mouse	WB	Cell Death Differ; Loss of ferroportin induces memory impairment by promoting ferroptosis in Alzheimer's disease.; Authors - Wen-Dai Bao