GPX1 Polyklonaler Antikörper

GPX1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 29329-1-AP

Synonyme

glutathione peroxidase 1, GPx 1, GPX1, GSHPx 1, GSHPX1

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Galerie der Validierungsdaten

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  WB analysis using 29329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29329-1-AP (GPX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29329-1-AP (GPX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29329-1-AP (GPX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausnierengewebe, Rattennierengewebe, SMMC-7721-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 1 publications below

Produktinformation

29329-1-AP bindet in WB, IF, ELISA GPX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPX1 fusion protein Ag31101
• Vollständiger Name: glutathione peroxidase 1
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC000742
• Gene symbol: GPX1
• Gene ID (NCBI): 2876
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Gpx protein, known as selenoprotein, consists selenium in its catalytic site. Gpx1 is an abundant isoform, which involves in scavenging endogenous ROS using electron provided by reduced glutathione and ubiquitously expressed the cytoplasm and mitochondria of all cell types. (PMID: 30632027)

Publikationen

Species	Application	Title
mouse	WB	Mol Ther; CircRNA Samd4 induces cardiac repair after myocardial infarction by blocking mitochondria-derived ROS output.; Authors - Hao Zheng
human,mouse	WB,IF	Cell Death Discov; Allicin promotes functional recovery in ischemic stroke via glutathione peroxidase-1 activation of Src-Akt-Erk; Authors - Fei Zhuang ; KD Validated
mouse	WB	Molecules; Polygonatum cyrtonema Hua Polysaccharides Protect BV2 Microglia Relief Oxidative Stress and Ferroptosis by Regulating NRF2/HO-1 Pathway; Authors - Jiayu Li
rat	WB	Pharmaceuticals (Basel); Adrenomedullin Improves Cardiac Remodeling and Function in Obese Rats with Hypertension.; Authors - Pei Qian
rat	WB	Acta Diabetol; Adrenomedullin ameliorates palmitic acid-induced insulin resistance through PI3K/Akt pathway in adipocytes.; Authors - Hang-Bing Dai
mouse	WB	bioRxiv; WDR23 mediates NRF2 proteostasis and cytoprotective capacity in the hippocampus; Authors - Jiahui Liu