GPT / ALT1 Polyklonaler Antikörper

GPT / ALT1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 16897-1-AP

Synonyme

AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ ALT1, GPT1

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Galerie der Validierungsdaten

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  WB analysis using 16897-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16897-1-AP (GPT/ ALT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Mausmilzgewebe, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below

Produktinformation

16897-1-AP bindet in WB, ELISA GPT / ALT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPT / ALT1 fusion protein Ag10424
• Vollständiger Name: glutamic-pyruvate transaminase (alanine aminotransferase)
• Berechnetes Molekulargewicht: 496 aa, 55 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC018207
• Gene symbol: GPT
• Gene ID (NCBI): 2875
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

Publikationen

Species	Application	Title
	WB	Cell Metab; Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.; Authors - Qiuying Chen
mouse	WB	Cell Rep; T Cell Activation Depends on Extracellular Alanine.; Authors - Noga Ron-Harel
human	WB	Oncotarget; Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.; Authors - Santosh K Bharti
human, mouse	WB	J Proteome Res; Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.; Authors - Shizhen Qin
mouse	WB	Mediators Inflamm; Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury.; Authors - Shuaiwei Wang
human,mouse	WB	Cancer Med; Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.; Authors - Xiaoxiao Cai