GPR37/Pael-R Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GPR37/Pael-R Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 14820-1-AP

Synonyme

EDNRBL, ETBR LP 1, GPR37, GPR37/Pael-R, hET(B)R LP, PAELR, Pael-R

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 14820-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14820-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14820-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 14820-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14820-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3200
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IF: See 4 publications below

Produktinformation

14820-1-AP bindet in WB, IHC, IF, ELISA GPR37/Pael-R und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPR37/Pael-R fusion protein Ag6591
• Vollständiger Name: G protein-coupled receptor 37 (endothelin receptor type B-like)
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC040007
• Gene symbol: GPR37
• Gene ID (NCBI): 2861
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The orphan G protein-coupled receptor GPR37, also known as PAELR (parkin-associated endothelin receptor-like receptor) or ETBR-LP-1 (endothelin B receptor-like protein 1), is a 613 aa, multi-pass membrane protein predominantly expressed in the brain. It is a substrate of parkin (PARK2). When overexpressed in cells, GPR37 tends to become insoluble, unfolded, and ubiquitinated in vivo. Accumulation of the unfolded protein may lead to dopaminergic neuronal death in juvenile Parkinson disease (JPD). This antibody recognizes endogenous GPR37, which migrates as an approximately 50-55 kDa band in SDS-PAGE (PMID:17519329).

Publikationen

Species	Application	Title
human	WB	Mol Syst Biol; Systematic protein-protein interaction mapping for clinically relevant human GPCRs.; Authors - Kate Sokolina
mouse	WB	Proc Natl Acad Sci U S A; GPR37 and GPR37L1 are receptors for the neuroprotective and glioprotective factors prosaptide and prosaposin.; Authors - Meyer Rebecca C RC
human	WB	Oncogene; GPR37 promotes colorectal cancer liver metastases by enhancing the glycolysis and histone lactylation via Hippo pathway; Authors - Jiamin Zhou
mouse,human	WB,IF	EMBO Rep; Parkinson's disease-associated receptor GPR37 is an ER chaperone for LRP6.; Authors - Birgit S Berger ; KD Validated
rat	WB	Glia; Glio- and neuro-protection by prosaposin is mediated by orphan G-protein coupled receptors GPR37L1 and GPR37.; Authors - Beihui Liu
mouse	WB	Biomedicines; Osteocalcin Alleviates Lipopolysaccharide-Induced Acute Inflammation via Activation of GPR37 in Macrophages.; Authors - Zhengjiang Qian ; KO Validated