GPR177 Polyklonaler Antikörper

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• KD/KO Validated

GPR177 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 17950-1-AP

Synonyme

C1orf139, EVI, G protein coupled receptor 177, GPR177, MRP, Protein wntless homolog, Sprinter, WLS, Wntless

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 17950-1-AP

  WB result of GPR177 antibody (17950-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPR177 transfected HEK-293 cells.

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  WB analysis using 17950-1-AP

  Various cell lysates were subjected to SDS PAGE followed by western blot with 17950-1-AP (GPR177 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17950-1-AP (GPR177 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17950-1-AP

  IP Result of anti-GPR177 (IP:17950-1-AP, 3ug; Detection:17950-1-AP 1:500) with mouse liver tissue lysate 12500ug.

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  IHC staining of human ovary using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse liver using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17950-1-AP (GPR177 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse liver using 17950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17950-1-AP (GPR177 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-87 MG-Zellen, HEK-293-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Eierstockgewebe, humanes Nierengewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 3 publications below

Produktinformation

17950-1-AP bindet in WB, IP, IHC, ELISA GPR177 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPR177 fusion protein Ag12428
• Vollständiger Name: G protein-coupled receptor 177
• Berechnetes Molekulargewicht: 541 aa, 62 kDa
• Beobachtetes Molekulargewicht: 46-65 kDa
• GenBank-Zugangsnummer: BC110826
• Gene symbol: GPR177
• Gene ID (NCBI): 79971
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).

Publikationen

Species	Application	Title
human	WB	Cell Res; Comprehensive profiling reveals mechanisms of SOX2-mediated cell fate specification in human ESCs and NPCs.; Authors - Chenlin Zhou
rat	WB	Front Physiol; A Longitudinal Study of T2 Mapping Combined With Diffusion Tensor Imaging to Quantitatively Evaluate Tissue Repair of Rat Skeletal Muscle After Frostbite.; Authors - Yue Gao
human	WB,IHC	Onco Targets Ther; Wntless (Wls): A Prognostic Index for Progression and Patient Survival of Breast Cancer.; Authors - Dandan Zheng
mouse	IHC	Reprod Sci; WNT5A Enhances LH-Mediated Expression of HAS2 in Granulosa Cells.; Authors - Qun Niu
mouse	WB	Int Ophthalmol; The effect of Wnt/β-catenin pathway on the scleral remolding in the mouse during form deprivation.; Authors - Shuyu Hu
goat	IHC	Cells; Integrative Analysis of Methylome and Transcriptome Reveals the Regulatory Mechanisms of Hair Follicle Morphogenesis in Cashmere Goat.; Authors - Shanhe Wang