GPD2 Monoklonaler Antikörper

GPD2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C12E2

Kat-Nr. : 68174-1-Ig

Synonyme

GDH2, GPD M, GPD2, GPDH M, GPDM, mGPDH, mtGPD

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Galerie der Validierungsdaten

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  WB analysis using 68174-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 68174-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68174-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, 4T1-Zellen, HeLa-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68174-1-Ig bindet in WB, ELISA GPD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GPD2 fusion protein Ag11212
• Vollständiger Name: glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
• Berechnetes Molekulargewicht: 41 kDa, 81 kDa
• GenBank-Zugangsnummer: BC019874
• Gene symbol: GPD2
• Gene ID (NCBI): 2820
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPD2(Glycerol-3-phosphate dehydrogenase, mitochondrial) belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.It is phosphorylated only in capacitated hamster spermatozoa and is noncanonically localized in the acrosome and principal piece in human, mouse, rat, and hamster spermatozoa, though in somatic cells it is localized in the mitochondria. This noncanonical localization may imply a role of GPD2 in acrosome reaction and hyperactivation(PMID:19333995 ).