GPD1L Polyklonaler Antikörper

GPD1L Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 17263-1-AP

Synonyme

GPD1 L, GPD1L, KIAA0089

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Galerie der Validierungsdaten

• 

  WB analysis of mouse heart using 17263-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17263-1-AP (GPD1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 17263-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17263-1-AP (GPD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17263-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17263-1-AP (GPD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17263-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17263-1-AP (GPD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HEK-293-Zellen, humanes Hirngewebe, humanes Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below

Produktinformation

17263-1-AP bindet in WB, ELISA GPD1L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPD1L fusion protein Ag10977
• Vollständiger Name: glycerol-3-phosphate dehydrogenase 1-like
• Berechnetes Molekulargewicht: 351 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC028726
• Gene symbol: GPD1L
• Gene ID (NCBI): 23171
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycerol-3-phosphate dehydrogenase 1-like (GPD1L) is encoded by the gene GPD1L, which is located on chromosome 3p22.3. GPD1L is responsible for catalyzing the conversion of sn-glycerol 3-phosphate to glycerone phosphate. GPD1L displayed a dysregulated state in a wide variety of cancers.

Publikationen

Species	Application	Title
human	WB	Cell Metab; Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD+ to alleviate mitochondrial disease.; Authors - Shanshan Liu
human	WB	Proc Natl Acad Sci U S A; A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria.; Authors - Shiqi Wu
rat	WB	J Transl Med; Atrial myocyte-derived exosomal microRNA contributes to atrial fibrosis in atrial fibrillation; Authors - Hongting Hao
human	WB	Cell Death Dis; A miR-210-3p regulon that controls the Warburg effect by modulating HIF-1α and p53 activity in triple-negative breast cancer.; Authors - Ye Du
human,mouse	WB	Mol Cell; Uncoupled glycerol-3-phosphate shuttle in kidney cancer reveals that cytosolic GPD is essential to support lipid synthesis; Authors - Cong-Hui Yao ; KD Validated
human	WB	Mol Cell; Saturation of the mitochondrial NADH shuttles drives aerobic glycolysis in proliferating cells; Authors - Yahui Wang