GOT2 Monoklonaler Antikörper

GOT2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Huhn, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B6C6

Rezensionen (1)

Kat-Nr. : 67738-1-Ig

Synonyme

FABP 1, FABPpm, Fatty acid binding protein, FLJ40994, GOT2, KAT4, KATIV, mAspAT, mitAAT, Transaminase A

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Galerie der Validierungsdaten

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  WB analysis using 67738-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67738-1-Ig (GOT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67738-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67738-1-Ig (GOT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 67738-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 67738-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 67738-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 67738-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, A431-Zellen, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, Rattenherzgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67738-1-Ig bindet in WB, IHC, ELISA GOT2 und zeigt Reaktivität mit Hausschwein, Huhn, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GOT2 fusion protein Ag6600
• Vollständiger Name: glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 39-40 kDa
• GenBank-Zugangsnummer: BC000525
• Gene symbol: GOT2
• Gene ID (NCBI): 2806
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sampath Kumar (Verified Customer) (02-26-2024)

The mouse GOT2 antibody worked consistently well at 1:6000 dilution on Avian skeletal muscle (pectoralis) homogenates. 6ug of total protein per sample was blotted on 0.2um nitrocellulose and probed with GOT2 primary antibody diluted in 5% milk. Anti-mouse secondary antibody at 1:10000 along with ECL prime provided optimum results.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Avian skeletal muscle (Pectoralis)

GOT2 Antibody Western Blot validation (1:5000 dilution) in Avian skeletal muscle (Pectoralis) (Cat no:67738-1-Ig)