GOSR2/Membrin Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

GOSR2/Membrin Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

7E7C7

Kat-Nr. : 66134-1-Ig

Synonyme

27 kDa Golgi SNARE protein, Bos1, GOSR2, GS27, Membrin

---

Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 66134-1-Ig

  WB result of GOSR2/Membrin antibody (66134-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOSR2/Membrin transfected Jurkat cells.

• 

  WB analysis using 66134-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66134-1-Ig (GOSR2/Membrin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66134-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66134-1-Ig (GOSR2/Membrin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66134-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66134-1-Ig (GOSR2/Membrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66134-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66134-1-Ig (GOSR2/Membrin antibody at dilution of 1:50.

• 

  IHC staining of human liver using 66134-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66134-1-Ig (GOSR2/Membrin antibody at dilution of 1:50.

• 

  IF Staining of human liver cancer using 66134-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GOSR2/Membrin antibody (66134-1-Ig, Clone: 7E7C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Leberkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66134-1-Ig bindet in WB, IHC, IF, ELISA GOSR2/Membrin und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GOSR2/Membrin fusion protein Ag21393
• Vollständiger Name: golgi SNAP receptor complex member 2
• Berechnetes Molekulargewicht: 212 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC009710
• Gene symbol: GOSR2
• Gene ID (NCBI): 9570
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the solube NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in Golgi apparatus. It is involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.

Publikationen

Species	Application	Title
human	WB	J Cell Sci; Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.; Authors - Dolma Choezom