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  • Featured Product
  • KD/KO Validated

GORASP2 Polyklonaler Antikörper

GORASP2 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-10598

Synonyme

DKFZp434D156, FLJ13139, Golgi phosphoprotein 6, GOLPH6, GORASP2, GRASP55, GRS2, p59

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated GORASP2 Polyclonal antibod (CL594-10598)

    IF Staining of HepG2 using CL594-10598

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 GORASP2 antibody (CL594-10598) at dilution of 1:600, CL488-Phalloidin (green).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated GORASP2 Polyclonal antibod (CL594-10598)

    FC experiment of HepG2 using CL594-10598

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GORASP2 (CL594-10598) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:300-1:1200
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-10598 bindet in IF, FC (Intra) GORASP2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen GORASP2 fusion protein Ag0950
Vollständiger Name golgi reassembly stacking protein 2, 55kDa
Berechnetes Molekulargewicht 55 kDa
Beobachtetes Molekulargewicht 59 kDa
GenBank-ZugangsnummerBC007770
Gene symbol GORASP2
Gene ID (NCBI) 26003
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 GORASP2 antibody CL594-10598Protokoll herunterladen
Standard-Protokolle
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