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GORASP2 Monoklonaler Antikörper

GORASP2 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C9A3

Kat-Nr. : CL488-66627

Synonyme

DKFZp434D156, FLJ13139, Golgi phosphoprotein 6, GOLPH6, GORASP2, GRASP55, GRS2, p59

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated GORASP2 Monoclonal a (CL488-66627)

    IF Staining of HepG2 using CL488-66627

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 GORASP2 antibody (CL488-66627, Clone: 1C9A3 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated GORASP2 Monoclonal a (CL488-66627)

    FC experiment of HepG2 using CL488-66627

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GORASP2 (CL488-66627, Clone:1C9A3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66627 bindet in IF, FC (Intra) GORASP2 und zeigt Reaktivität mit Hausschwein, human, Ratten

Getestete Reaktivität Hausschwein, human, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen GORASP2 fusion protein Ag17607
Vollständiger Name golgi reassembly stacking protein 2, 55kDa
Berechnetes Molekulargewicht 55 kDa
Beobachtetes Molekulargewicht 59 kDa
GenBank-ZugangsnummerBC007770
Gene symbol GORASP2
Gene ID (NCBI) 26003
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 GORASP2 antibody CL488-66627Protokoll herunterladen
FC protocol for CL Plus 488 GORASP2 antibody CL488-66627Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen