GOLGA3 Polyklonaler Antikörper

GOLGA3 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 21193-1-AP

Synonyme

GCP170, GOLGA3, Golgin 160, Golgin subfamily A member 3, MEA 2

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Galerie der Validierungsdaten

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  WB analysis of L02 using 21193-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21193-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21193-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 21193-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 21193-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21193-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 21193-1-AP

  Immunofluorescent analysis of HepG2 cells, using GOLGA3 antibody 21193-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Herzgewebe, humanes Hodengewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 1 publications below

Produktinformation

21193-1-AP bindet in WB, IF, ELISA GOLGA3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GOLGA3 fusion protein Ag15531
• Vollständiger Name: golgi autoantigen, golgin subfamily a, 3
• Berechnetes Molekulargewicht: 1498 aa, 167 kDa
• Beobachtetes Molekulargewicht: 167 kDa
• GenBank-Zugangsnummer: BC142658
• Gene symbol: GOLGA3
• Gene ID (NCBI): 2802
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production.; Authors - Gunjan Joshi
human	WB	PLoS Biol; A  conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation.; Authors - Weichao Zhang
human	WB	J Biol Chem; GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin.; Authors - Erpan Ahat
human	WB	Mol Biol Cell; Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.; Authors - Stephen C Ireland
human,mouse	WB,IF	PeerJ; Mutation of S461, in the GOLGA3 phosphorylation site, does not affect mouse spermatogenesis; Authors - Changtong Xu
human	WB	PLoS One; The mechanism on phosphorylation of Hsp20Ser16 inhibit GA stress and ER stress during OGD/R.; Authors - Tonglin Lu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (04-02-2023)

Very good for WB.

• Applications: Western Blot