GOLGA2/GM130 Polyklonaler Antikörper

GOLGA2/GM130 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-11308

Synonyme

Cell and organelle markers, GM130, GM130 autoantigen, GOLGA2, GOLGA2/GM130, Golgi Marker, Golgin 95, Golgin subfamily A member 2

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Galerie der Validierungsdaten

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  IF Staining of HeLa using CL488-11308

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 GOLGA2/GM130 antibody (CL488-11308) at dilution of 1:200, CoraLite®594 Coilin antibody (CL594-10967, red).

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  IF Staining of HepG2 using CL488-11308

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 GOLGA2/GM130 antibody (CL488-11308) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-11308

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GOLGA2/GM130 (CL488-11308) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-11308 bindet in IF, FC (Intra) GOLGA2/GM130 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GOLGA2/GM130 fusion protein Ag1848
• Vollständiger Name: golgi autoantigen, golgin subfamily a, 2
• Berechnetes Molekulargewicht: 111 kDa
• GenBank-Zugangsnummer: BC014188
• Gene symbol: GOLGA2
• Gene ID (NCBI): 2801
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggests that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C