GNPNAT1 Polyklonaler Antikörper

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• KD/KO Validated

GNPNAT1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 16282-1-AP

Synonyme

GNA1, GNPNAT, GNPNAT1, Gpnat1, Phosphoglucosamine acetylase

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Galerie der Validierungsdaten

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  WB analysis using 16282-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16282-1-AP (GNPNAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse large intestine using 16282-1-AP

  mouse large intestine tissue were subjected to SDS PAGE followed by western blot with 16282-1-AP (GNPNAT1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse large intestine using 16282-1-AP

  IP Result of anti-GNPNAT1 (IP:16282-1-AP, 3ug; Detection:16282-1-AP 1:300) with mouse large intestine tissue lysate 3200ug.

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  IHC staining of human liver using 16282-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16282-1-AP (GNPNAT1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16282-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16282-1-AP (GNPNAT1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, Maus-Dickdarmgewebe, Maus-Kolongewebe, MDA-MB-231-Zellen
• Erfolgreiche IP: Maus-Dickdarmgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

16282-1-AP bindet in WB, IP, IHC, ELISA GNPNAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNPNAT1 fusion protein Ag9375
• Vollständiger Name: glucosamine-phosphate N-acetyltransferase 1
• Berechnetes Molekulargewicht: 184 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21-23 kDa
• GenBank-Zugangsnummer: BC012179
• Gene symbol: GNPNAT1
• Gene ID (NCBI): 64841
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucosamine-6 phosphate-N-acetyl Transferase (GNPNAT1) is a key enzyme in the hexosamine biosynthetic pathway. It converts D-glucosamine 6-phosphate to N-acetyl-D-glucosamine 6-phosphate, the acetylation step allowing carbon and nitrogen enter into the hexosamine biosynthetic pathway. Recently inhibition of GNPNAT1 has been reported to promote proliferation and aggressiveness of castration-resistant prostate cancer. (27194471) GNPNAT1 is a small dimeric protein localized in the Golgi and endosome membranes.

Publikationen

Species	Application	Title
human	WB	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
human	WB,IHC	Nat Commun; Inhibition of the hexosamine biosynthetic pathway promotes castration-resistant prostate cancer.; Authors - Akash K Kaushik ; KD Validated
human	IHC	Front Mol Biosci; Upregulation of GNPNAT1 Predicts Poor Prognosis and Correlates With Immune Infiltration in Lung Adenocarcinoma.; Authors - Wenting Liu
human	WB	Front Immunol; GNPNAT1 is a potential biomarker correlated with immune infiltration and immunotherapy outcome in breast cancer; Authors - Renjie Yuan
mouse	WB	Int J Mol Sci; ER Stress-Perturbed Intracellular Protein O-GlcNAcylation Aggravates Podocyte Injury in Diabetes Nephropathy; Authors - Shicong Song