GNPAT Monoklonaler Antikörper

GNPAT Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

2E5C10

Kat-Nr. : 66453-1-Ig

Synonyme

DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66453-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 66453-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66453-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66453-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66453-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66453-1-Ig bindet in WB, IHC,ELISA GNPAT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GNPAT fusion protein Ag6740
• Vollständiger Name: glyceronephosphate O-acyltransferase
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 65-69 kDa
• GenBank-Zugangsnummer: BC000450
• Gene symbol: GNPAT
• Gene ID (NCBI): 8443
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS. (PMID: 21990100) This antibody recognize the 65-69 kDa GNPAT protein.