GNPAT Polyklonaler Antikörper

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• KD/KO Validated

GNPAT Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 14931-1-AP

Synonyme

DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 14931-1-AP

  WB result of GNPAT antibody (14931-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNPAT transfected HepG2 cells.

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  WB analysis of HepG2 using 14931-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14931-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 14931-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 14931-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14931-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14931-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14931-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 14931-1-AP

  IP Result of anti-GNPAT (IP:14931-1-AP, 4ug; Detection:14931-1-AP 1:500) with COLO 320 cells lysate 2400ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, COLO 320-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, Maushirngewebe, PC-3-Zellen, Transfizierte HEK-293-Zellen
• Erfolgreiche IP: COLO 320-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Produktinformation

14931-1-AP bindet in WB, IP, IHC, IF, ELISA GNPAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNPAT fusion protein Ag6735
• Vollständiger Name: glyceronephosphate O-acyltransferase
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 65-69 kDa
• GenBank-Zugangsnummer: BC000450
• Gene symbol: GNPAT
• Gene ID (NCBI): 8443
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS. (PMID:21990100) This antibody recognize the 69 kDa GNPAT protein.

Publikationen

Species	Application	Title
human	WB	Cell Host Microbe; Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication.; Authors - Pierre M Jean Beltran ; KD Validated
mouse	WB	J Clin Invest; Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission.; Authors - Hongsuk Park
human	IF	Int J Mol Sci; Differential Expression of Peroxisomal Proteins in Distinct Types of Parotid Gland Tumors.; Authors - Malin Tordis Meyer
human,hamster	WB	Hum Mutat; Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3.; Authors - Itzkovitz Brandon B
mouse,rat	WB,IHC,IF	Front Cell Dev Biol; Plasmalogens Regulate Retinal Connexin 43 Expression and Müller Glial Cells Gap Junction Intercellular Communication and Migration.; Authors - Rémi Karadayi ; KD Validated
human	IF	Histochem Cell Biol; C22-bronchial and T7-alveolar epithelial cell lines of the immortomouse are excellent murine cell culture model systems to study pulmonary peroxisome biology and metabolism.; Authors - Srikanth Karnati