GNMT Polyklonaler Antikörper

GNMT Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 18790-1-AP

Synonyme

glycine N methyltransferase, GNMT

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Galerie der Validierungsdaten

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  WB analysis of rat liver using 18790-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18790-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 18790-1-AP

  IP result of anti-GNMT(IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 1840 ug.

• 

  IP experiment of mouse liver using 18790-1-AP

  IP result of anti-GNMT(IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 3040 ug.

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  IHC staining of human prostate hyperplasia using 18790-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 18790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 18790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, LNCaP-Zellen, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Prostatahyperplasie-Gewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 22 publications below
• IHC: See 1 publications below

Produktinformation

18790-1-AP bindet in WB, IP, IHC, ELISA GNMT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNMT fusion protein Ag4598
• Vollständiger Name: glycine N-methyltransferase
• Berechnetes Molekulargewicht: 295 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC032627
• Gene symbol: GNMT
• Gene ID (NCBI): 27232
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).

Publikationen

Species	Application	Title
mouse	WB	Mol Metab; Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction.; Authors - Ferrol I Rome
human	IHC	Int J Mol Sci; MAT2A Localization and Its Independently Prognostic Relevance in Breast Cancer Patients.; Authors - Pei-Yi Chu
	WB	J Anim Sci Biotechnol; Dietary betaine activates hepaticVTGIIexpression in laying hens associated with hypomethylation ofGRgene promoter and enhancedGRexpression.; Authors - Nagmeldin A Omer
human	WB	Mol Oncol; Elevated N-methyltransferase expression induced by hepatic stellate cells contributes to the metastasis of hepatocellular carcinoma via regulation of the CD44v3 isoform.; Authors - Jie Li
rat	WB	J Agric Food Chem; Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.; Authors - Shu Yang
pig	WB	J Agric Food Chem; Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.; Authors - Yimin Jia