GNE Polyklonaler Antikörper

GNE Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 25079-1-AP

Synonyme

DMRV, GLCNE, GNE, IBM2, ManAc kinase, NM, Uae1, UDP GlcNAc 2 epimerase

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Galerie der Validierungsdaten

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  WB analysis using 25079-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25079-1-AP (GNE antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25079-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25079-1-AP (GNE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 25079-1-AP

  IP Result of anti-GNE (IP:25079-1-AP, 4ug; Detection:25079-1-AP 1:500) with Jurkat cells lysate 3600ug.

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  IHC staining of human kidney using 25079-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25079-1-AP (GNE Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 25079-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25079-1-AP (GNE Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 25079-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25079-1-AP (GNE Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 25079-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25079-1-AP (GNE Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Jurkat-Zellen, Rattenlebergewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

25079-1-AP bindet in WB, IP, IHC, IF, ELISA GNE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNE fusion protein Ag18689
• Vollständiger Name: glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
• Berechnetes Molekulargewicht: 722 aa, 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC121179
• Gene symbol: GNE
• Gene ID (NCBI): 10020
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The bifunctional enzyme UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE) is essential for early embryonic development and catalyzes the rate limiting step in sialic acid biosynthesis. It plays an important role in neuronal cell and brain development, and is strongly involved in cardiac tissue and skeletal muscle early survival and organization.. This protein has 5 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Neoplasma; miR-550a-5p promotes the proliferation and migration of hepatocellular carcinoma by targeting GNE via the Wnt/β-catenin signaling pathway; Authors - Chun-Ming Li
human	WB	Cell Rep; Efficient generation of isogenic primary human myeloid cells using CRISPR-Cas9 ribonucleoproteins.; Authors - Joseph Hiatt
mouse	WB,IF,IP	Front Genome Ed; In vivo and in vitro genome editing to explore GNE functions; Authors - Nili Ilouz ; KO Validated
human	IHC	Cells; Loss of GNE Predicts Lymph Node Metastasis in Early Gastric Cancer; Authors - Xinying Guo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (03-15-2022)

Works well @ 1:50 for IHC on FFPE prostate tissue and 1:2000 for WB on LNCaP cells

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate