GNA13 Monoklonaler Antikörper

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• KD/KO Validated

GNA13 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

2G8F10

Rezensionen (1)

Kat-Nr. : 67188-1-Ig

Synonyme

G alpha 13, G protein subunit alpha 13, G13, GNA13

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Galerie der Validierungsdaten

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  WB analysis using 67188-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67188-1-Ig (GNA13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67188-1-Ig

  WB result of GNA13 antibody (67188-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNA13 transfected HEK-293 cells.

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  IHC staining of human breast cancer using 67188-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67188-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67188-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67188-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 67188-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GNA13 antibody (67188-1-Ig, Clone: 2G8F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Hausschwein-Hirngewebe, HepG2-Zellen, Maushirngewebe, PC-12-Zellen, Rattenhirngewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

67188-1-Ig bindet in WB, IHC, IF, CoIP, ELISA GNA13 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GNA13 fusion protein Ag7316
• Vollständiger Name: guanine nucleotide binding protein (G protein), alpha 13
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC036756
• Gene symbol: GNA13
• Gene ID (NCBI): 10672
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Integrin β3 directly inhibits the Gα13-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis; Authors - Yaping Zhang
human	WB,CoIP	J Proteome Res; Proteomic Investigation of Differential Interactomes of Glypican 1 and a Putative Disease-Modifying Variant of Ataxia; Authors - Nur Cengiz Winter

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (12-29-2023)

Worked well for WB at 1:1000 in Glioma cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioma