GMPS Polyklonaler Antikörper

GMPS Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 16376-1-AP

Synonyme

Glutamine amidotransferase, GMP synthetase, GMPS

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 16376-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16376-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 16376-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16376-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16376-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16376-1-AP

  IP Result of anti-GMPS (IP:16376-1-AP, 4ug; Detection:16376-1-AP 1:800) with HeLa cells lysate 1080ug.

• 

  IHC staining of human liver cancer using 16376-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16376-1-AP (GMPS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 16376-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16376-1-AP (GMPS antibody) at dilution of 1:100 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HL-60-Zellen, Jurkat-Zellen, Maushirngewebe, Maushodengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below

Produktinformation

16376-1-AP bindet in WB, IP, IHC, ELISA GMPS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GMPS fusion protein Ag9350
• Vollständiger Name: guanine monphosphate synthetase
• Berechnetes Molekulargewicht: 693 aa, 77 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC012178
• Gene symbol: GMPS
• Gene ID (NCBI): 8833
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
human	WB, IHC	PLoS Biol; Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.; Authors - Yajing Lv
human	WB	Aging (Albany NY); The upregulated expression of RFC4 and GMPS mediated by DNA copy number alteration is associated with the early diagnosis and immune escape of ESCC based on a bioinformatic analysis.; Authors - Jing Wang
human	WB,IHC	J Biomed Sci; TRIM21-SERPINB5 aids GMPS repression to protect nasopharyngeal carcinoma cells from radiation-induced apoptosis.; Authors - Panpan Zhang
mouse	WB	Cell Rep; Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.; Authors - Nicole M Sayles