GMPPB Polyklonaler Antikörper

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• KD/KO Validated

GMPPB Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15094-1-AP

Synonyme

GMPPB, KIAA1851

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 15094-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15094-1-AP (GMPPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15094-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15094-1-AP (GMPPB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 15094-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 15094-1-AP (GMPPB antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

15094-1-AP bindet in WB, IHC, IF, ELISA GMPPB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GMPPB fusion protein Ag7168
• Vollständiger Name: GDP-mannose pyrophosphorylase B
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40-43 kDa
• GenBank-Zugangsnummer: BC001141
• Gene symbol: GMPPB
• Gene ID (NCBI): 29925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GMPPB is a cytoplasmic enzyme that catalyzes the formation of GDP-mannose, a key substrate for several glycosylation pathways, including O-mannosylation of α-DG. GMPPB facilitates the formation of the sugar donor GDP-mannose and pyrophosphate from mannose-1-phosphate and GTP, a very early step in the glycosylation pathway (PMID: 37834154, 27147698). GMPPB mutations correlate with several congenital diseases, including severe congenital muscular dystrophy (CMD) with abnormalities in the brain and eye (PMID: 26310427, 30684953). Western blot analysis detected GMPPB at an apparent molecular mass of 40 kDa.

Publikationen

Species	Application	Title
human	WB	iScience; A Trace Amount of Galactose, a Major Component of Milk Sugar, Allows Maturation of Glycoproteins during Sugar Starvation.; Authors - Norio Sasaoka
mouse	WB	Front Mol Biosci; Altered Glycosylation in the Aging Heart.; Authors - Patricia Franzka
zebrafish	WB	Mol Biomed; GMPPB-congenital disorders of glycosylation associate with decreased enzymatic activity of GMPPB.; Authors - Zhe Liu
human,mouse	WB	Front Nutr; Altered mannose metabolism in chronic stress and depression is rapidly reversed by vitamin B12; Authors - Patricia Franzka
human	WB,IHC	Int J Mol Sci; Silencing GMPPB Inhibits the Proliferation and Invasion of GBM via Hippo/MMP3 Pathways; Authors - Zi-Lu Huang ; KD Validated