GMDS Polyklonaler Antikörper

GMDS Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15442-1-AP

Synonyme

GDP D mannose dehydratase, GDP mannose 4,6 dehydratase, GMD, GMDS, SDR3E1

---

Galerie der Validierungsdaten

• 

  WB analysis using 15442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 15442-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15442-1-AP (GMDS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15442-1-AP (GMDS antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15442-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15442-1-AP (GMDS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, HeLa-Zellen, Rattenmilzgewebe, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IP: See 1 publications below

Produktinformation

15442-1-AP bindet in WB, IP, IHC, IF, ELISA GMDS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GMDS fusion protein Ag7319
• Vollständiger Name: GDP-mannose 4,6-dehydratase
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 40-42 kDa
• GenBank-Zugangsnummer: BC000117
• Gene symbol: GMDS
• Gene ID (NCBI): 2762
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GDP-D-mannose-4,6-dehydratase (GMDS) is the key enzyme in the 'de novo' pathway of GDP-L-fucose biosynthesis(PMID:10462046). The enzyme is also named as GMD and belongs to the GDP-mannose 4,6-dehydratase family. The gene encodes a 42 kDa protein that catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor.

Publikationen

Species	Application	Title
human	WB,IP	Mol Cell Biol; GDP-mannose-4,6-dehydratase is a cytosolic partner of tankyrase 1 that inhibits its poly(ADP-ribose) polymerase activity.; Authors - Bisht Kamlesh K KK
human	WB	Mol Cell Proteomics; Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei.; Authors - Lilli Saarinen
human	WB	Mol Cell Proteomics; Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei; Authors - Lilli Saarinen
human	WB	Cell Chem Biol; High-Sensitivity and Low-Toxicity Fucose Probe for Glycan Imaging and Biomarker Discovery.; Authors - Yasuhiko Kizuka