GOLGA2/GM130 Monoklonaler Antikörper

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• KD/KO Validated

GOLGA2/GM130 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

2A4F11

Kat-Nr. : 66662-1-Ig

Synonyme

Cell and organelle markers, GM130, GM130 autoantigen, GOLGA2, Golgi Marker, Golgin 95, Golgin subfamily A member 2

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66662-1-Ig

  WB result of GOLGA2/GM130 antibody (66662-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HEK-293 cells.

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  WB analysis of HeLa using 66662-1-Ig

  WB result of GOLGA2/GM130 antibody (66662-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells.

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  WB analysis using 66662-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66662-1-Ig (GOLGA2/GM130 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66662-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66662-1-Ig (GM130;GOLGA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66662-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (66662-1-Ig, Clone: 2A4F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

66662-1-Ig bindet in WB, IF, ELISA GOLGA2/GM130 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GOLGA2/GM130 fusion protein Ag1848
• Vollständiger Name: golgi autoantigen, golgin subfamily a, 2
• Berechnetes Molekulargewicht: 111 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC014188
• Gene symbol: GOLGA2
• Gene ID (NCBI): 2801
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Publikationen

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu
human	WB	Mol Metab; CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.; Authors - Han Zeng
human,rat	WB	Front Mol Biosci; Schwann Cell-Derived Exosomes Induce the Differentiation of Human Adipose-Derived Stem Cells Into Schwann Cells.; Authors - Nan Zhou
human	WB	Anticancer Drugs; Exosomal circRNA Scm-like with four malignant brain tumor domains 2 (circ-SFMBT2) enhances the docetaxel resistance of prostate cancer via the microRNA-136-5p/tribbles homolog 1 pathway; Authors - Xiaoliang Tan
human	WB	Nat Commun; Identification of indocyanine green as a STT3B inhibitor against mushroom α-amanitin cytotoxicity; Authors - Bei Wang
human	WB	J Mater Sci Mater Med; Enhanced therapeutic effects of mesenchymal stem cell-derived extracellular vesicles within chitosan hydrogel in the treatment of diabetic foot ulcers; Authors - Shuangshuang Yang