GLUT2 Monoklonaler Antikörper

GLUT2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1G8B1

Kat-Nr. : 66889-1-Ig

Synonyme

GLUT 2, GLUT2, SLC2A2

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Galerie der Validierungsdaten

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  WB analysis of 4T1 using 66889-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66889-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66889-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66889-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 66889-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66889-1-Ig (GLUT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66889-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66889-1-Ig (GLUT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 4T1-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

66889-1-Ig bindet in WB, IHC, IF, ELISA GLUT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GLUT2 fusion protein Ag14551
• Vollständiger Name: solute carrier family 2 (facilitated glucose transporter), member 2
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC060041
• Gene symbol: SLC2A2
• Gene ID (NCBI): 6514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLUT2 is a member of glucose transporters or GLUTs which catalyze the glucose transport across plasma membrane. GLUT2 is selectively expressed in hepatocytes, pancreatic beta cells, and absorptive renal and intestinal epithelial cells. GLUT2 is required to maintain normal glucose homeostasis and normal function and development of the endocrine pancreas. Its absence leads to symptoms characteristic of non-insulin-dependent diabetes mellitus.

Publikationen

Species	Application	Title
mouse	IF	Sci Rep; Regulatory mechanisms of glucose absorption in the mouse proximal small intestine during fasting and feeding; Authors - Chisato Nakamura
human	IHC	Oncol Lett; Prediction and verification of the prognostic biomarker SLC2A2 and its association with immune infiltration in gastric cancer; Authors - Weijian Zhang