GLUD1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GLUD1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

39

Kat-Nr. : 14299-1-AP

Synonyme

GDH, GDH 1, GDH1, GLUD, GLUD1, glutamate dehydrogenase 1

---

Galerie der Validierungsdaten

• 

  WB analysis using 14299-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14299-1-AP (GLUD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 14299-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14299-1-AP (GLUD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14299-1-AP

  IP Result of anti-GLUD1 (IP:14299-1-AP, 4ug; Detection:14299-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human gliomas using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, L02-Zellen, Mauslebergewebe, Mauslungengewebe, PC-3-Zellen, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Lebergewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 35 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14299-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA GLUD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLUD1 fusion protein Ag5694
• Vollständiger Name: glutamate dehydrogenase 1
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC040132
• Gene symbol: GLUD1
• Gene ID (NCBI): 2746
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.

Publikationen

Species	Application	Title
		Science; Metabolic recycling of ammonia via glutamate dehydrogenase supports breast cancer biomass.; Authors - Jessica B Spinelli
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
human	WB	Cell; Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.; Authors - Wen Yang
human	WB	Cancer Cell; Non-oncogene Addiction to SIRT3 Plays a Critical Role in Lymphomagenesis.; Authors - Meng Li
mouse	WB	Nat Metab; Gut microbiome-derived ammonia modulates stress vulnerability in the host; Authors - Pei Wang
mouse	WB	Nat Metab; Quantitative analysis of metabolic fluxes in brown fat and skeletal muscle during thermogenesis; Authors - Grace Park