GLTSCR2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GLTSCR2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 27353-1-AP

Synonyme

GLT, GLTSCR2, NOP53, p60, PICT 1, PICT1

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Galerie der Validierungsdaten

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  WB analysis of human placenta using 27353-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27353-1-AP (GLTSCR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 27353-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27353-1-AP (GLTSCR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27353-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27353-1-AP (GLTSCR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 27353-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27353-1-AP (GLTSCR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 27353-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27353-1-AP (GLTSCR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 27353-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GLTSCR2 antibody (27353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

27353-1-AP bindet in WB, IHC, IF, ELISA GLTSCR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLTSCR2 fusion protein Ag26358
• Vollständiger Name: glioma tumor suppressor candidate region gene 2
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC010095
• Gene symbol: GLTSCR2
• Gene ID (NCBI): 29997
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Biochem Biophys Res Commun; MTR4 adaptor PICT1 functions in two distinct steps during pre-rRNA processing; Authors - Sotaro Miyao ; KD Validated
human		bioRxiv; Nucleolar dynamics are determined by the ordered assembly of the ribosome; Authors - Jessica Sheu-Gruttadauria
human	WB	Dev Cell; Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method; Authors - Sarah Carden

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (03-15-2022)

U2OS cells were fixed in 4% PFA for 15' at Room temperature. Cells were washed once with PBS for. Membrane permeabilisation was then performed with 0.3% Triton in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at room temperature. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature). In this picture, cells are stained for Hoechst (DNA marker, in magenta) and GLTSCR2 (nucleolar protein in yellow).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: U2OS (Human Osteosarcoma) cells

GLTSCR2 Antibody Immunofluorescence validation (1:200 dilution) in U2OS (Human Osteosarcoma) cells (Cat no:27353-1-AP)