GLRA1 Polyklonaler Antikörper

GLRA1 Polyklonal Antikörper für FC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 17951-1-AP

Synonyme

GLRA1, glycine receptor, alpha 1, STHE

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 17951-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17951-1-AP (GLRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17951-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17951-1-AP (GLRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 17951-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 17951-1-AP (GLRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17951-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17951-1-AP (GLRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  FC experiment of SH-SY5Y using 17951-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug GLRA1 antibody (17951-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

17951-1-AP bindet in WB, IF, FC, CoIP, ELISA GLRA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLRA1 fusion protein Ag12431
• Vollständiger Name: glycine receptor, alpha 1
• Berechnetes Molekulargewicht: 449 aa, 52 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC074980
• Gene symbol: GLRA1
• Gene ID (NCBI): 2741
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Publikationen

Species	Application	Title
mouse	CoIP	PLoS Biol; mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.; Authors - Zi-Yang Zhang
mouse	WB,IF	J Biol Chem; Clinical, genetic, and functional characterization of the glycine receptor β-subunit A455P variant in a family affected by hyperekplexia syndrome.; Authors - Ghada I Aboheimed
mouse	WB	Psychopharmacology (Berl); Contribution of skeletal muscular glycine to rapid antidepressant effects of ketamine in an inflammation-induced mouse model of depression.; Authors - Niannian Huang
mouse	WB	Eur J Pharmacol; Functional expression of glycine receptors in DRG neurons of mice.; Authors - Lin Yao
human	WB	Neuropharmacology; Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn.; Authors - Zi-Yang Zhang