GLO1 Polyklonaler Antikörper

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• KD/KO Validated

GLO1 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (1)

Kat-Nr. : 15140-1-AP

Synonyme

Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, glyoxalase I, Ketone aldehyde mutase, Lactoylglutathione lyase, Methylglyoxalase

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Galerie der Validierungsdaten

• 

  WB analysis using 15140-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15140-1-AP (GLO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15140-1-AP

  IP Result of anti-GLO1 (IP:15140-1-AP, 4ug; Detection:15140-1-AP 1:1000) with HepG2 cells lysate 1000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HeLa-Zellen, HepG2-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 1 publications below

Produktinformation

15140-1-AP bindet in WB, IP, IF, ELISA GLO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLO1 fusion protein Ag7314
• Vollständiger Name: glyoxalase I
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21-25 kDa
• GenBank-Zugangsnummer: BC001741
• Gene symbol: GLO1
• Gene ID (NCBI): 2739
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glo1 is known to play a role in many diseases including diabetes mellitus, Alzheimer's disease, and cancer. The molecular mass of the dimer was 46 kDa determined by gel permeation chromatography.

Publikationen

Species	Application	Title
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
mouse	WB	Front Pharmacol; Protective effect of heat-processed Gynostemma pentaphyllum on high fat diet-induced glucose metabolic disorders mice; Authors - Jin-Bo Xie
mouse	WB,IF	Phytomedicine; Protective effects of Huang-Lian-Jie-Du Decoction on diabetic nephropathy through regulating AGEs/RAGE/Akt/Nrf2 pathway and metabolic profiling in db/db mice.; Authors - Dan Tang
human	WB	Oncotarget; Glyoxalase 1 gene is highly expressed in basal-like human breast cancers and contributes to survival of ALDH1-positive breast cancer stem cells.; Authors - Shoma Tamori ; KD Validated
mouse	WB	Life Sci Alliance; Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.; Authors - Shengya Tian
rat	WB	Free Radic Biol Med; Methylglyoxal accumulation contributes to accelerated brain aging in spontaneously hypertensive rats; Authors - Wen-Yuan Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Billie (Verified Customer) (07-10-2021)

Strong band at around 25 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 with 10ug protein loaded
• Cell Tissue Type: Astrocytes