Alpha Galactosidase A Monoklonaler Antikörper

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• KD/KO Validated

Alpha Galactosidase A Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2B2C5

Kat-Nr. : 66121-1-Ig

Synonyme

Alpha D galactosidase A, Alpha galactosidase A, galactosidase, alpha

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66121-1-Ig

  WB result of GLA antibody (66121-1-Ig, 1:2000) with si-Control and si-GLA transfected HEK293 cells..

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  WB analysis using 66121-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of LNCaP using 66121-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66121-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66121-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66121-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 66121-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 66121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 66121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 66121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 66121-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Nierengewebe, LNCaP-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:10000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66121-1-Ig bindet in WB, IHC, IF, ELISA Alpha Galactosidase A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Alpha Galactosidase A fusion protein Ag7505
• Vollständiger Name: galactosidase, alpha
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC002689
• Gene symbol: GLA
• Gene ID (NCBI): 2717
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLA, also named as Melibiase and Alpha-galactosidase A, belongs to the glycosyl hydrolase 27 family. It hydrolyzes terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Fabry disease is an X-linked lysosomal storage disorder resulting from the deficient activity of GLA. Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis; Authors - Sebastian Boland