GGPS1 Polyklonaler Antikörper

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• KD/KO Validated

GGPS1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 14944-1-AP

Synonyme

GGPP synthase, GGPPS, GGPPS1, GGPPSase, GGPS1

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 14944-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14944-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14944-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse bladder using 14944-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14944-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14944-1-AP

  IP Result of anti-GGPS1 (IP:14944-1-AP, 4ug; Detection:14944-1-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human kidney using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14944-1-AP (GGPS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14944-1-AP (GGPS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14944-1-AP (GGPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14944-1-AP (GGPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14944-1-AP (GGPS1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14944-1-AP (GGPS1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Herzgewebe, humanes Hodengewebe, humanes Plazenta-Gewebe, Maus-Blasengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hodengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Produktinformation

14944-1-AP bindet in WB, IP, IHC, IF, FC, ELISA GGPS1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GGPS1 fusion protein Ag6745
• Vollständiger Name: geranylgeranyl diphosphate synthase 1
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC067768
• Gene symbol: GGPS1
• Gene ID (NCBI): 9453
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GGPS1(Geranylgeranyl pyrophosphate synthase) belongs to the FPP/GGPP synthase family and catalyzes the synthesis of GGPP, an important molecule responsible for the C20-prenylation of protein and for the regulation of a nuclear hormone receptor. GGPP synthase puri¢ed from bovine brain is reported to be a homo-oligomer (150-195 kDa) with a molecular mass of 37.5 kDa for the monomer. This is consistent with the calculated molecular mass (35 kDa) of mouse and human GGPP synthases (PMID:10101267).

Publikationen

Species	Application	Title
mouse	IF	Dev Cell; Quantitative proteomic profiling identifies global protein network dynamics in murine embryonic heart development; Authors - Whitney Edwards
mouse	WB	FEBS J; Conditional Loss of Geranylgeranyl Diphosphate Synthase Alleviates Acute Obstructive Cholestatic Liver Injury by Regulating Hepatic Bile Acid Metabolism.; Authors - Wen-Jun Jia
	IHC	Am J Pathol; Geranylgeranyl Diphosphate Synthase Modulates Fetal Lung Branching Morphogenesis Possibly through Controlling K-Ras Prenylation.; Authors - Wen-Jun Jia
human	WB,IHC	J Cell Mol Med; Overexpression of geranylgeranyl diphosphate synthase contributes to tumour metastasis and correlates with poor prognosis of lung adenocarcinoma.; Authors - Xiaoxia Wang ; KD Validated
mouse	WB,IF	Cells; Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model.; Authors - Natalie Längst
human	WB	J Hepatocell Carcinoma; miR-612 Enhances RSL3-Induced Ferroptosis of Hepatocellular Carcinoma Cells via Mevalonate Pathway; Authors - Kailin Xing