GGCX Polyklonaler Antikörper

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• KD/KO Validated

GGCX Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 16209-1-AP

Synonyme

gamma glutamyl carboxylase, GC, GGCX, VKCFD1

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 16209-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16209-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16209-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16209-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16209-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 16209-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16209-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16209-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16209-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16209-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16209-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 16209-1-AP

  IP Result of anti-GGCX (IP:16209-1-AP, 4ug; Detection:16209-1-AP 1:2000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human liver using 16209-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16209-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16209-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16209-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16209-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16209-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HEK-293-Zellen, HepG2-Zellen, L02-Zellen, Mauslebergewebe, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Produktinformation

16209-1-AP bindet in WB, IP, IHC, ELISA GGCX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GGCX fusion protein Ag9185
• Vollständiger Name: gamma-glutamyl carboxylase
• Berechnetes Molekulargewicht: 758 aa, 88 kDa
• Beobachtetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: BC013979
• Gene symbol: GGCX
• Gene ID (NCBI): 2677
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GGCX(Gamma-glutamyl carboxylase) is also named as GC and belongs to the vitamin K-dependent gamma-carboxylase family. This 94 kDa (including all modifications, such as the five N-linked glycosylations), is a 5-pass transmembrane protein and a key regulator of blood coagulation(PMID:20518534). It mediates the vitamin K-dependent carboxylation of glutamate residues to calcium-binding gamma-carboxyglutamate (Gla) residues with the concomitant conversion of the reduced hydroquinone form of vitamin K to vitamin K epoxide. Defects in GGCX are a cause of combined deficiency of vitamin K-dependent clotting factors type 1 (VKCFD1) and pseudoxanthoma elasticum-like disorder with multiple coagulation factor deficiency (PXEL-MCFD)(PMID:9845520;17110937).

Publikationen

Species	Application	Title
mouse	WB	J Cell Biol; GGCX and VKORC1 inhibit osteocalcin endocrine functions.; Authors - Mathieu Ferron
		Blood; Characterization of vitamin K-dependent carboxylase mutations that cause bleeding and nonbleeding disorders.; Authors - Jian-Ke Tie
human	WB	Methods Enzymol; Functional Study of the Vitamin K Cycle Enzymes in Live Cells.; Authors - J-K Tie ; KO Validated
human	WB	JCI Insight; VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo.; Authors - Julie Lacombe
human	WB	Oncotarget; Divergent effects of vitamins K1 and K2 on triple negative breast cancer cells.; Authors - Sarah Beaudin
human,mouse	WB,IP	Cell Rep; Vitamin K-dependent carboxylation regulates Ca2+ flux and adaptation to metabolic stress in β cells; Authors - Julie Lacombe