GGCT Polyklonaler Antikörper

GGCT Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 16257-1-AP

Synonyme

C7orf24, CRF21, Gamma glutamylcyclotransferase, GCTG, Ggc, GGCT

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Galerie der Validierungsdaten

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  WB analysis using 16257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16257-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16257-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 16257-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16257-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 16257-1-AP

  IP result of anti-GGCT (IP:16257-1-AP, 4ug; Detection:16257-1-AP 1:300) with mouse kidney tissue lysate 3000 ug.

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  IHC staining of mouse small intestine using 16257-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16257-1-AP (GGCT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 16257-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16257-1-AP (GGCT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16257-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16257-1-AP (GGCT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Hirngewebe, Jurkat-Zellen, Mauslebergewebe, Mausnierengewebe, U-937-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

16257-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA GGCT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GGCT fusion protein Ag9284
• Vollständiger Name: gamma-glutamyl cyclotransferase
• Berechnetes Molekulargewicht: 188 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21-25 kDa
• GenBank-Zugangsnummer: BC019243
• Gene symbol: GGCT
• Gene ID (NCBI): 79017
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	FASEB J; Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.; Authors - Kenneth N Maclean
human	WB	Sci Rep; Identification of proteins responsible for adriamycin resistance in breast cancer cells using proteomics analysis.; Authors - Zhipeng Wang
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Integrated application of transcriptome and metabolomics reveals potential therapeutic targets for the polarization of atherosclerotic macrophages; Authors - Lin Shen
human	WB,IHC,CoIP	Int J Mol Sci; Interaction of MRPL9 and GGCT Promotes Cell Proliferation and Migration by Activating the MAPK/ERK Pathway in Papillary Thyroid Cancer; Authors - Hui-Min Zhang
mouse	WB,IHC	Redox Biol; Unbalanced redox status network as an early pathological event in congenital cataracts; Authors - Eloy Bejarano