GFRA2 Polyklonaler Antikörper

GFRA2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 21973-1-AP

Synonyme

GDNF family receptor alpha 2, GDNF receptor alpha 2, GDNF receptor beta, GDNFR alpha 2, GDNFR beta, GDNFRB, GFR alpha 2, GFRA2, Neurturin receptor alpha, NRTNR ALPHA, NTNR alpha, NTNRA, RET ligand 2, RETL2, TRNR2

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 21973-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21973-1-AP (GFRA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21973-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21973-1-AP (GFRA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21973-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21973-1-AP (GFRA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 21973-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21973-1-AP (GFRA2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Multizellen/-gewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

21973-1-AP bindet in WB, IHC,ELISA GFRA2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GFRA2 fusion protein Ag16893
• Vollständiger Name: GDNF family receptor alpha 2
• Berechnetes Molekulargewicht: 464 aa, 52 kDa
• Beobachtetes Molekulargewicht: 34-40 kDa, 50-55 kDa
• GenBank-Zugangsnummer: BC041688
• Gene symbol: GFRA2
• Gene ID (NCBI): 2675
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GFRA2, also named as GDNFRB, RETL2 and TRNR2, belongs to the GDNFR family. It is a receptor for neurturin. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It is a potent survival factor for central dopaminergic neurons, motor neurons, and several other populations of neurons in the central and peripheral nervous systems. The GDNF signal is mediated by a complex of the receptor tyrosine kinase RET and a glycosylphosphatidylinositol (GPI)-linked protein, GDNFRA. GFRA2 has three isoforms with MW 34-36 kDa, 39-41 kDa and 50-55 kDa. This antibody recognizes all the three isoforms.

Publikationen

Species	Application	Title
human	IHC	Cancer Lett; GFRα2 prompts cell growth and chemoresistance through down-regulating tumor suppressor gene PTEN via Mir-17-5p in pancreatic cancer.; Authors - Jiangning Gu
rat	WB	Biotechnol Appl Biochem; Ellagic acid supports neuron by regulating astroglia Nrf2.; Authors - Guo-Qing Wang