GFI1 Polyklonaler Antikörper

GFI1 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 14198-1-AP

Synonyme

GFI 1, GFI1, Zinc finger protein 163, Zinc finger protein Gfi 1, ZNF163

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 14198-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14198-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 14198-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14198-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14198-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14198-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14198-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14198-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14198-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HL-60-Zellen, Maus-Thymusgewebe, PC-3-Zellen, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

14198-1-AP bindet in WB, IHC, ELISA GFI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GFI1 fusion protein Ag5401
• Vollständiger Name: growth factor independent 1 transcription repressor
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 62-66 kDa
• GenBank-Zugangsnummer: BC032751
• Gene symbol: GFI1
• Gene ID (NCBI): 2672
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GFI is a transcriptional repressor that is transiently induced during T-cell differentiation. It has critical roles in enhancing T helper-2 (Th2) cell expansion and in repressing induction of Th17 and CD103-positive inducible regulatory T (Treg) cells [PMID: 19188499]. GFI1 transcriptional regulator oncoprotein plays a crucial role in hematopoietic, inner ear, and pulmonary neuroendocrine cell development and governs cell processes as diverse as self-renewal of hematopoietic stem cells, proliferation, apoptosis, differentiation, cell fate specification, and oncogenesis (PMID: 18805794). The calculated molecular weight of GFI1 protein is about 45 kDa, but the modification of ubiquitinated GIF1 protein is about 62-66 kDa (PMID: 12721361).

Publikationen

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda