GEN1 Monoklonaler Antikörper

GEN1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A10D10

Kat-Nr. : 68060-1-Ig

Synonyme

DKFZp781F0986, FLJ40869, Gen, GEN1

---

Galerie der Validierungsdaten

• 

  WB analysis of U2OS using 68060-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 68060-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68060-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, Daudi-Zellen, K-562-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68060-1-Ig bindet in WB, ELISA GEN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GEN1 fusion protein Ag31247
• Vollständiger Name: Gen homolog 1, endonuclease (Drosophila)
• Berechnetes Molekulargewicht: 103 kDa
• Beobachtetes Molekulargewicht: 98-100 kDa
• GenBank-Zugangsnummer: NM_182625
• Gene symbol: GEN1
• Gene ID (NCBI): 348654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GEN1 is a endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. GEN1 efficiently cleaves both single and double HJs contained within large recombination intermediates.