GEFT-Specific Polyklonaler Antikörper

GEFT-Specific Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Rezensionen (1)

Kat-Nr. : 14839-1-AP

Synonyme

ARHGEF25, GEFT, GEFT-Specific, p63RhoGEF, RhoA/RAC/CDC42 exchange factor

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Galerie der Validierungsdaten

• 

  WB analysis of mouse skeletal muscle using 14839-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14839-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14839-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14839-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14839-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14839-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14839-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14839-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14839-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14839-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14839-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14839-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14839-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14839-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14839-1-AP (GEFT-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14839-1-AP

  IP Result of anti-GEFT-Specific (IP:14839-1-AP, 4ug; Detection:14839-1-AP 1:1500) with mouse brain tissue lysate 8000ug.

• 

  IHC staining of human pancreas using 14839-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.

• 

  IHC staining of human pancreas using 14839-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.

• 

  IHC staining of human skeletal muscle using 14839-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.

• 

  IHC staining of human pancreas using 14839-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.

• 

  IHC staining of human pancreas using 14839-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.

• 

  IHC staining of human skeletal muscle using 14839-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 14839-1-AP (GEFT-Specific Antibody) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, HEK-293-Zellen, Mausherzgewebe, Mauslebergewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

14839-1-AP bindet in WB, IP, IHC, IF, ELISA GEFT-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: RhoA/RAC/CDC42 exchange factor
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: NM_182947
• Gene symbol: GEFT
• Gene ID (NCBI): 115557
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68 kDa).

Publikationen

Species	Application	Title
mouse	WB	Genes Dev; TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway.; Authors - Kant Shashi S
human	WB	Circ Res; p63RhoGEF couples Gα(q/11)-mediated signaling to Ca2+ sensitization of vascular smooth muscle contractility.; Authors - Momotani Ko K
mouse	WB	Sci Signal; RSK2 contributes to myogenic vasoconstriction of resistance arteries by activating smooth muscle myosin and the Na+/H+ exchanger.; Authors - Mykhaylo V Artamonov
rat	WB,IHC	FASEB J; p63RhoGEF--a key mediator of angiotensin II-dependent signaling and processes in vascular smooth muscle cells.; Authors - Wuertz Christina M CM
mouse	WB,IHC,IF	Mol Cell Biol; Modulation of muscle regeneration, myogenesis, and adipogenesis by the Rho family guanine nucleotide exchange factor GEFT.; Authors - Bryan Brad A BA
human	WB	J Hypertens; Increased level of p63RhoGEF and RhoA/Rho kinase activity in hypertensive patients.; Authors - Lorenzo A Calò

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joelle (Verified Customer) (09-10-2019)

Overnight at 4 degrees

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HHL16

GEFT-Specific Antibody Western Blot, validation (1/1000 dilution) in HHL16 (Cat no:14839-1-AP)