GEFT Monoklonaler Antikörper

GEFT Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

5A11F8

Kat-Nr. : 66012-1-Ig

Synonyme

ARHGEF25, GEFT, p63RhoGEF, RhoA/RAC/CDC42 exchange factor

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Galerie der Validierungsdaten

• 

  WB analysis of mouse skeletal muscle using 66012-1-Ig

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 66012-1-Ig

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66012-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66012-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66012-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66012-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 66012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 66012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 66012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 66012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66012-1-Ig bindet in WB, IHC,ELISA GEFT und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GEFT fusion protein Ag10819
• Vollständiger Name: RhoA/RAC/CDC42 exchange factor
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 53 kDa, 63 kDa
• GenBank-Zugangsnummer: BC012860
• Gene symbol: GEFT
• Gene ID (NCBI): 115557
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68kDa).

Publikationen

Species	Application	Title
human	WB	Cancer Sci; Inhibition of heat shock protein 90 destabilizes receptor tyrosine kinase ROR1 in lung adenocarcinoma.; Authors - Behnoush Khaledian