GDI2 Monoklonaler Antikörper

GDI2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

4B8F3

Kat-Nr. : 60078-1-Ig

Synonyme

GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, RABGDIB

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Galerie der Validierungsdaten

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  WB analysis using 60078-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60078-1-Ig (GDI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HepG2 using 60078-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60078-1-Ig (GDI2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 60078-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 60078-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 60078-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 60078-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 60078-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 60078-1-Ig (GDI2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 60078-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GDI2 antibody (60078-1-Ig, Clone: 4B8F3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 60078-1-Ig

  Immunofluorescent analysis of Hela cells, using GDI2 antibody 60078-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60078-1-Ig bindet in WB, IHC, IF, ELISA GDI2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GDI2 fusion protein Ag4613
• Vollständiger Name: GDP dissociation inhibitor 2
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC005145
• Gene symbol: GDI2
• Gene ID (NCBI): 2665
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.