GDI1 Monoklonaler Antikörper

GDI1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

4E1G1

Kat-Nr. : 66434-1-Ig

Synonyme

GDI 1, GDI1, GDIL, GDP dissociation inhibitor 1, MRX41, MRX48, Oligophrenin 2, OPHN2, Protein XAP 4, Rab GDI alpha, RABGD1A, RABGDIA, XAP 4, XAP4

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Galerie der Validierungsdaten

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  WB analysis of U-251 using 66434-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 66434-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66434-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66434-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66434-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66434-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66434-1-Ig (GDI1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66434-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66434-1-Ig (GDI1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 66434-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66434-1-Ig (GDI1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-251-Zellen, HEK-293-Zellen, Maushirngewebe, Neuro-2a-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

66434-1-Ig bindet in WB, IHC, IF, ELISA GDI1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GDI1 fusion protein Ag17511
• Vollständiger Name: GDP dissociation inhibitor 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC000317
• Gene symbol: GDI1
• Gene ID (NCBI): 2664
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55 kDa protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034)

Publikationen

Species	Application	Title
human	IHC	Bioengineered; Overexpression of GDP dissociation inhibitor 1 gene associates with the invasiveness and poor outcomes of colorectal cancer; Authors - Xiao Xie
human	IHC	Signal Transduct Target Ther; Erianin, a novel dibenzyl compound in Dendrobium extract, inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis.; Authors - Peng Chen