GDF15 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GDF15 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 27455-1-AP

Synonyme

GDF 15, GDF15, MIC 1, MIC1, NAG 1, NRG 1, NSAID activated gene 1 protein, NSAID regulated gene 1 protein, PDF, PLAB, Placental TGF beta, PTGFB

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Galerie der Validierungsdaten

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  WB analysis using 27455-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27455-1-AP (GDF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27455-1-AP

  WB result of GDF15 antibody (27455-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GDF15 transfected HepG2 cells.

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  WB analysis using 27455-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 27455-1-AP (GDF15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 27455-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27455-1-AP (GDF15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 27455-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27455-1-AP (GDF15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 27455-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27455-1-AP (GDF15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 27455-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 27455-1-AP (GDF15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 27455-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 27455-1-AP (GDF15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, LNCaP-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, Maus-Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 12 publications below
• WB: See 22 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

27455-1-AP bindet in WB, IHC, IF, ELISA GDF15 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GDF15 fusion protein Ag26760
• Vollständiger Name: growth differentiation factor 15
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC008962
• Gene symbol: GDF15
• Gene ID (NCBI): 9518
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.

Publikationen

Species	Application	Title
human,mouse	WB	Gastroenterology; Hepatic Stellate Cells in Hepatocellular Carcinoma Promote Tumor Growth Via Growth Differentiation Factor 15 Production.; Authors - Yuta Myojin ; KO Validated
human	WB	Nat Commun; Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer.; Authors - Tiziano Bernasocchi
human,mouse	WB,IHC	Cancer Lett; SULF2 enhances GDF15-SMAD axis to facilitate the initiation and progression of pancreatic cancer.; Authors - Ruizhe He
rat	WB,IF	Oxid Med Cell Longev; Cyclic Polypeptide D7 Protects Bone Marrow Mesenchymal Cells and Promotes Chondrogenesis during Osteonecrosis of the Femoral Head via Growth Differentiation Factor 15-Mediated Redox Signaling.; Authors - Jiazheng Chen
human	WB	Cancers (Basel); GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.; Authors - Chiara Bellio ; KD Validated
human	WB	Antioxidants (Basel); Grape Seed Proanthocyanidins Induce Apoptosis and Cell Cycle Arrest of HepG2 Cells Accompanied by Induction of the MAPK Pathway and NAG-1.; Authors - Lihua Wang