GCK Monoklonaler Antikörper

GCK Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1C3A3

Kat-Nr. : 67216-1-Ig

Synonyme

GCK, GK, GLK, Glucokinase, glucokinase (hexokinase 4), Hexokinase 4, Hexokinase D, Hexokinase type IV, HHF3, HK IV, HK4, HKIV, HXKP, MODY2

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Galerie der Validierungsdaten

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  WB analysis of pig liver using 67216-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67216-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67216-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 67216-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67216-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 67216-1-Ig

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 67216-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67216-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67216-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67216-1-Ig (GCK antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67216-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67216-1-Ig (GCK antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 67216-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 67216-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Lebergewebe, HepG2-Zellen, L02-Zellen, Mauslebergewebe, Rattenlebergewebe, SMMC-7721-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Leberkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:300-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67216-1-Ig bindet in WB, IHC, IF, ELISA GCK und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GCK fusion protein Ag8116
• Vollständiger Name: glucokinase (hexokinase 4)
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC001890
• Gene symbol: GCK
• Gene ID (NCBI): 2645
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa.

Publikationen

Species	Application	Title
human,mouse	WB	Front Pharmacol; Berberrubine, a Main Metabolite of Berberine, Alleviates Non-Alcoholic Fatty Liver Disease via Modulating Glucose and Lipid Metabolism and Restoring Gut Microbiota; Authors - Sa Yang