GCK Polyklonaler Antikörper

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• KD/KO Validated

GCK Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 19666-1-AP

Synonyme

GCK, GK, GLK, Glucokinase, glucokinase (hexokinase 4), Hexokinase 4, Hexokinase D, Hexokinase type IV, HHF3, HK IV, HK4, HKIV, HXKP, MODY2

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Galerie der Validierungsdaten

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  WB analysis using 19666-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19666-1-AP (GCK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 19666-1-AP

  IP result of anti-GCK(IP:19666-1-AP, 4ug; Detection:19666-1-AP 1:4000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of human liver using 19666-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19666-1-AP (GCK Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 19666-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19666-1-AP (GCK Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Produktinformation

19666-1-AP bindet in WB, IP, IHC, IF, FC, ELISA GCK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GCK fusion protein Ag8116
• Vollständiger Name: glucokinase (hexokinase 4)
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC001890
• Gene symbol: GCK
• Gene ID (NCBI): 2645
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa.

Publikationen

Species	Application	Title
mouse	WB	Sci Transl Med; Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes; Authors - Hitoshi Watanabe ; KD Validated
mouse	WB,FC	Nat Commun; Citrullination of glucokinase is linked to autoimmune diabetes.; Authors - Mei-Ling Yang
mouse	WB	PLoS Biol; Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.; Authors - Xuxiao He
mouse	WB	iScience; Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions; Authors - Xinghong Guo
	WB	Free Radic Biol Med; Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.; Authors - Kelei Dong
mouse	WB	J Genet Genomics; Functionalized Gadofullerene Ameliorates Impaired Glycolipid Metabolism in Type 2 Diabetic Mice.; Authors - Jin Wu